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beta-2-Microglobulin Polyclonal Antibody, HRP

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Datasheet
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Questions & Answers
beta-2-Microglobulin Polyclonal Antibody, HRP

Product Details

200-403-141-0100

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:200-1:1,000
-

ELISA (ELISA)

1:2,000-1:10,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
View immunogen

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% gentamicin sulfate

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.

Target Information

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-F; B2-microglobulin; Beta 2 microglobin; Beta 2 microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m

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Gene Aliases: B2M; CDABP0092; HDCMA22P; IMD43

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UniProt ID: (Human) P61769

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Entrez Gene ID: (Human) 567

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Function(s)
glycoprotein binding protein binding identical protein binding
Process(es)
retina homeostasis positive regulation of T cell mediated cytotoxicity antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent positive regulation of T cell cytokine production regulation of membrane depolarization negative regulation of neuron projection development antibacterial humoral response antigen processing and presentation of endogenous peptide antigen via MHC class I positive regulation of protein binding T cell differentiation in thymus protein refolding response to drug cellular protein metabolic process innate immune response response to cadmium ion positive regulation of receptor-mediated endocytosis regulation of defense response to virus by virus regulation of immune response defense response to Gram-negative bacterium defense response to Gram-positive bacterium iron ion homeostasis interferon-gamma-mediated signaling pathway cellular response to lipopolysaccharide cellular response to iron ion negative regulation of receptor binding positive regulation of receptor binding positive regulation of ferrous iron import into cell positive regulation of ferrous iron binding positive regulation of transferrin receptor binding
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