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Invitrogen
beta-2 Defensin Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
beta-2 Defensin Antibody (500-P161GBT-25UG) in ELISA
Product Details
500-P161GBT-25UG
Species Reactivity
Published species
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Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BD-2. Anti-Human BD-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-2 (500-P161G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-2.
Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P161GBT-1MG will be provided as 2 x 500 µg
Target Information
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; Defensin beta 2; Defensin beta 4A; Defensin beta2; Defensin, beta 2; defensin, beta 4; defensin, beta 4A; Defensin-beta2; SAP1; Skin-antimicrobial peptide 1
Gene Aliases: BD-2; DEFB-2; DEFB102; DEFB2; DEFB4; DEFB4A; DEFB4B; HBD-2; SAP1
UniProt ID: (Human) O15263
Entrez Gene ID: (Human) 1673
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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