Rockland

alpha-Tubulin Monoclonal Antibody (17H11.F10)

Name: alpha-Tubulin Monoclonal Antibody (17H11.F10)
Brand: Rockland
SKU: 200-301-880

View all (82) alpha Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (13)

alpha-Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

alpha-Tubulin Antibody (200-301-880) in ICC/IF

Immunofluorescence microscopy of Anti-tubulin in U-87 MG cells using FITC-conjugated Fluorescent TrueBlot® anti-mouse IgG (p/n 18-0217-32) for detection. U87-MG cells were fixed with 100% methanol, permeabilized with 0.3% Triton X-100, blocked with 5% rat serum/0.3 Triton X-100 for 1 hr, then incubated with 15 µg/mL of anti-a-tubulin primary antibody (p/n 200-301-880) at 4°C overnight. Following 3 washes in... View More

alpha-Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha-Tubulin Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image alpha-Tubulin Monoclonal Antibody (17H11.F10)

Product Details

200-301-880

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

Immunocytochemistry (ICC/IF)

0.1 µg/mL

ELISA (ELISA)

1:300,000

Immunoprecipitation (IP)

1:1,000

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

17H11.F10

Immunogen

Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; Beta-tubulin class-II; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 6; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2 chain; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3; tubulin, alpha 3c; tubulin, alpha 3d; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta polypeptide; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; BOS_13022; BOS_16828; BOS_16831; BOS_2117; BOS_5053; BOS_5055; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBAT; TUBB; TUBB2; TUBB2A; TUBB2B