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  • Primary Antibodies ›
  • alpha-1d Adrenoceptor Antibodies

Alomone Labs, LTD

alpha 1D-Adrenergic Receptor (extracellular) Polyclonal Antibody

View all (5) alpha-1d Adrenoceptor antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite alpha 1D-Adrenergic Receptor (extracellular) Polyclonal Antibody

  • Antibody Testing Data (5)
alpha 1D-Adrenergic Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
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alpha 1D-Adrenergic Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
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alpha 1D-Adrenergic Receptor (extracellular) Antibody (AAR-019-200UL) in ICC/IF

Expression of alpha 1D-Adrenoceptorin rat PC12 cells - Cell surface detection of alpha 1D-Adrenoceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019) (1:100)followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Merge of A and live view of the cells. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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alpha 1D-Adrenergic Receptor (extracellular) Antibody in Immunocytochemistry (ICC/IF)
alpha 1D-Adrenergic Receptor (extracellular) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
alpha 1D-Adrenergic Receptor (extracellular) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
alpha 1D-Adrenergic Receptor (extracellular) Antibody in Western Blot (WB)
alpha 1D-Adrenergic Receptor (extracellular) Antibody in Flow Cytometry (Flow)
alpha 1D-Adrenergic Receptor (extracellular) Polyclonal Antibody

Product Details

AAR-019-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

5-10 µg/0.5-1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat alpha 1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S), 3rd extracellular loop
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

Target Information

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADA1D; adrenergic receptor delta1; adrenergic receptor, alpha 1d; adrenergic, alpha -1D-, receptor; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1D-, receptor; alpha 1D-adrenoceptor; alpha 1D-adrenoreceptor; Alpha-1A adrenergic receptor; Alpha-1D adrenergic receptor; Alpha-1D adrenoceptor; Alpha-1D adrenoreceptor; alpha-1D-adrenergic receptor; Alpha-adrenergic receptor 1a; Alpha1delta-AR; RA42

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Gene Aliases: [a]1d; Adra-1; ADRA1; ADRA1A; ADRA1D; ADRA1R; Adrd1; ALPHA1; alpha1D-AR; DAR; dJ779E11.2; Gpcr8; Spr8

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UniProt ID: (Human) P25100, (Rat) P23944

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Entrez Gene ID: (Human) 146, (Mouse) 11550, (Rat) 29413

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Function(s)
alpha1-adrenergic receptor activity protein binding signal transducer activity G-protein coupled receptor activity adrenergic receptor activity
Process(es)
negative regulation of the force of heart contraction involved in baroreceptor response to increased systemic arterial blood pressure norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure signal transduction G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling aging positive regulation of vasoconstriction positive regulation of smooth muscle contraction micturition adrenergic receptor signaling pathway adenylate cyclase-activating adrenergic receptor signaling pathway DNA metabolic process adenylate cyclase-modulating G-protein coupled receptor signaling pathway multicellular organism development cell proliferation positive regulation of cell proliferation
It has to be done as per old AB suggested Products section.
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