Invitrogen

XRCC4 Monoclonal Antibody (OTI4H9)

Name: XRCC4 Monoclonal Antibody (OTI4H9)
Brand: Invitrogen
SKU: MA5-24983

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View all (19) XRCC4 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (17)
Advanced Verification (1)

XRCC4 Antibody in Immunocytochemistry (ICC/IF)

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XRCC4 Antibody (MA5-24983) in ICC/IF

Immunofluorescence analysis of DNA repair protein XRCC4 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with XRCC4 Monoclonal Antibody (OTI4H9) (Product # MA5-24983) at 1:200 in 0.1% BSA, incubated at room temperature for 5 hours and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More

XRCC4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

XRCC4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with DNA repair protein XRCC4 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-XRCC4 Monoclonal Antibody (OTI4H9) (Product # MA5-24983). {KD}

Immunogen image

Immunogen Image XRCC4 Monoclonal Antibody (OTI4H9)

Product Details

MA5-24983

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4H9

Immunogen

Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2725686

Target Information

The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein XRCC4; hXRCC4; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross-complementing protein 4

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Gene Aliases: 2310057B22Rik; AW413319; AW545101; SSMED; XRCC4

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UniProt ID: (Human) Q13426, (Mouse) Q924T3

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Entrez Gene ID: (Human) 7518, (Mouse) 108138

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Function(s)

DNA binding protein binding protein C-terminus binding ligase activity

Process(es)

double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination response to X-ray protein sumoylation DNA ligation involved in DNA repair positive regulation of ligase activity cellular response to lithium ion establishment of integrated proviral latency in utero embryonic development pro-B cell differentiation DNA ligation DNA repair cellular response to DNA damage stimulus central nervous system development response to ionizing radiation response to gamma radiation T cell differentiation in thymus V(D)J recombination immunoglobulin V(D)J recombination negative regulation of neuron apoptotic process isotype switching positive regulation of fibroblast proliferation positive regulation of neurogenesis

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XRCC4 Monoclonal Antibody (OTI4H9)

XRCC4 Antibody (MA5-24983) in ICC/IF

Product Details

MA5-24983

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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