Invitrogen

WWTR1 Polyclonal Antibody

Name: WWTR1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-119927

View all (24) WWTR1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

WWTR1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

WWTR1 Antibody (PA5-119927) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of TAZ in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with WWTR1 Polyclonal Antibody (Product # PA5-119927) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI ... View More

WWTR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Immunogen image

Immunogen Image WWTR1 Polyclonal Antibody

Product Details

PA5-119927

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:200

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human TAZ aa 1-200/400

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2913499

Product Specific Information

Positive Control: Human placenta tissue lysate, 293T cell lysate, human kidney tissue, mouse brain tissue, A549, HT-29.

Subcellular Location: Cell membrane, Cytoplasm, Membrane, Nucleus.

Target Information

WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: DKFZp586I1419; FLJ27004; FLJ45718; Tafazzin; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1

View more View less

Gene Aliases: 2310058J06Rik; 2610021I22Rik; C78399; TAZ; WWTR1

View more View less

UniProt ID: (Human) Q9GZV5, (Mouse) Q9EPK5

View more View less

Entrez Gene ID: (Human) 25937, (Mouse) 97064

View more View less

Function(s)

transcription coactivator activity transcription corepressor activity protein binding protein homodimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of protein kinase activity positive regulation of cell proliferation positive regulation of epithelial to mesenchymal transition protein ubiquitination stem cell division SCF-dependent proteasomal ubiquitin-dependent protein catabolic process glomerulus development multicellular organism growth hippo signaling negative regulation of catenin import into nucleus negative regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter mesenchymal cell differentiation cilium morphogenesis regulation of SMAD protein import into nucleus regulation of canonical Wnt signaling pathway kidney morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation negative regulation of canonical Wnt signaling pathway regulation of nucleic acid-templated transcription transcription initiation from RNA polymerase II promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

WWTR1 Polyclonal Antibody

WWTR1 Antibody (PA5-119927) in ICC/IF

Product Details

PA5-119927

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!