Bioss

Vimentin Polyclonal Antibody

Name: Vimentin Polyclonal Antibody
Brand: Bioss
SKU: BS-23063R

View all (198) Vimentin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Vimentin Antibody (BS-23063R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded human cervical cancer section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-23063R) at 1:400 overnight at 4°C, followed by a conjugated secondary... View More

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Immunogen image

Immunogen Image Vimentin Polyclonal Antibody

Product Details

BS-23063R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Vimentin, amino acids 101-200.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Human) P08670, (Mouse) P20152, (Rat) P31000

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Entrez Gene ID: (Human) 7431, (Mouse) 22352, (Rat) 81818

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Function(s)

glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

positive regulation of gene expression negative regulation of neuron projection development astrocyte development intermediate filament-based process intermediate filament organization regulation of axonogenesis Bergmann glial cell differentiation positive regulation of glial cell proliferation SMAD protein signal transduction lens fiber cell development regulation of Schwann cell migration movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Vimentin Polyclonal Antibody

Vimentin Antibody (BS-23063R) in IHC (P)

Product Details

BS-23063R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

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