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  • VEGF Antibodies

Invitrogen

VEGF Recombinant Mouse Monoclonal Antibody (A.4.6.1)

View all (88) VEGF antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite VEGF Recombinant Mouse Monoclonal Antibody (A.4.6.1)

  • Antibody Testing Data (3)
VEGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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VEGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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VEGF Antibody (MA5-47926) in IHC (P)

Immunohistochemistry (Paraffin) analysis of VEGF in formaldehyde fixed, paraffin embedded rat brain tissue using VEGF Recombinant Monoclonal Antibody (Product # MA5-47926) at a concentration of 5 µg/mL (40x magnification). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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VEGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
VEGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
VEGF Antibody in Western Blot (WB)

Product Details

MA5-47926

Applications
Tested Dilution
Publications

Western Blot (WB)

1.0 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Inhibition Assays (IA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

A.4.6.1

Immunogen

Recombinant human VEGF.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074370

Product Specific Information

Specificity: Binds native and reduced human VEGF (isoform 165), with a Kd of 0.8 nm determined for the original clone.

Target Information

VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: L-VEGF; MGC70609; RP1-261G23.1; VAS; Vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; Vasculotropin; VEGF-A; VEGF1; VEGFMGC70609; VPF; VPF ant

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Gene Aliases: MVCD1; VEGF; VEGF-A; VEGF164; VEGFA; VPF

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UniProt ID: (Human) P15692, (Rat) P16612

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Entrez Gene ID: (Human) 7422, (Rat) 83785

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Function(s)
fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding receptor binding neuropilin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization platelet degranulation positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth eye photoreceptor cell development positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly primitive erythrocyte differentiation mammary gland alveolus development cardiac vascular smooth muscle cell development coronary artery morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein kinase D signaling blood vessel development vasculature development lymphangiogenesis blood vessel remodeling sprouting angiogenesis basophil chemotaxis regulation of transcription from RNA polymerase II promoter female pregnancy aging cell proliferation response to cold positive regulation of signal transduction cell migration negative regulation of cell-cell adhesion cell differentiation hyaluronan metabolic process response to estradiol response to progesterone response to vitamin A cellular response to drug vascular endothelial growth factor signaling pathway wound healing T-helper 1 type immune response negative regulation of programmed cell death positive regulation of vascular permeability negative regulation of neuron apoptotic process endothelial cell migration cellular response to fibroblast growth factor stimulus regulation of endothelial cell differentiation negative regulation of bone resorption mRNA stabilization lung alveolus development blood vessel morphogenesis positive regulation of smooth muscle cell proliferation neuron development response to folic acid positive regulation of protein kinase B signaling cell chemotaxis lung vasculature development positive regulation of mast cell chemotaxis angiogenesis involved in coronary vascular morphogenesis positive regulation of ERK1 and ERK2 cascade response to oxygen levels response to dexamethasone cellular response to dexamethasone stimulus negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of protein localization to early endosome negative regulation of extrinsic apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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