Invitrogen

UBC9 Recombinant Rabbit Monoclonal Antibody (SC0534)

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Datasheet
Protocols
Questions & Answers
UBC9 Recombinant Rabbit Monoclonal Antibody (SC0534)

Product Details

MA5-32200

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC0534

Immunogen

Synthetic peptide within Human UBC9 aa 15-60
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809487

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mUBC9; OTTHUMP00000198131; p18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9); Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); Ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

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Gene Aliases: 5830467E05Rik; C358B7.1; F830028O17Rik; P18; UBC9; UbcE2A; Ubce2i; UBCE9; UBE2I

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UniProt ID: (Human) P63279, (Rat) P63281, (Mouse) P63280

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Entrez Gene ID: (Human) 7329, (Rat) 25573, (Mouse) 22196

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Function(s)
protein binding ATP binding transcription factor binding ligase activity SUMO transferase activity enzyme binding ubiquitin protein ligase binding HLH domain binding poly(A) RNA binding ubiquitin protein ligase activity SUMO conjugating enzyme activity RING-like zinc finger domain binding ubiquitin-protein transferase activity protein C-terminus binding bHLH transcription factor binding nucleotide binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process chromosome segregation mitotic nuclear division regulation of receptor activity protein ubiquitination protein sumoylation positive regulation of intracellular steroid hormone receptor signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of transcription, DNA-templated positive regulation of sequence-specific DNA binding transcription factor activity cell division positive regulation of SUMO transferase activity cellular protein modification process viral process global genome nucleotide-excision repair cell cycle multicellular organism development
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