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Invitrogen

UBC9 Recombinant Rabbit Monoclonal Antibody (SAIC-36A-9)

View all (17) UBC9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite UBC9 Recombinant Rabbit Monoclonal Antibody (SAIC-36A-9)

  • Antibody Testing Data (2)
UBC9 Antibody in Immunocytochemistry (ICC/IF)
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UBC9 Antibody in Immunocytochemistry (ICC/IF)
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UBC9 Antibody (MA5-47917) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of UBC9 in Jurkat cells using UBC9 Recombinant Monoclonal Antibody (Product # MA5-47917). Paraformaldehyde-fixed cells were immobilized on a coverslip and stained with UBC9 Recombinant Monoclonal Antibody at a 1:100 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom UBC9 Recombinant Monoclonal Antibody, DAPI,... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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UBC9 Antibody in Immunocytochemistry (ICC/IF)
UBC9 Antibody in Flow Cytometry (Flow)
UBC9 Recombinant Rabbit Monoclonal Antibody (SAIC-36A-9)

Product Details

MA5-47917

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

Assay-dependent
-

Mass Spectrometry (MS)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SAIC-36A-9

Immunogen

Peptide "DHPFGFVAVPTK", derived from the SUMO-conjugating enzyme UBC9, conjugated to KLH.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074361

Product Specific Information

Specificity: Recognises human UBE2I.

Target Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000198131; p18; RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

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Gene Aliases: C358B7.1; P18; UBC9; UBCE9; UBE2I

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UniProt ID: (Human) P63279

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Entrez Gene ID: (Human) 7329

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Function(s)
protein binding ATP binding transcription factor binding ligase activity SUMO transferase activity enzyme binding ubiquitin protein ligase binding HLH domain binding poly(A) RNA binding ubiquitin protein ligase activity SUMO conjugating enzyme activity RING-like zinc finger domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cellular protein modification process ubiquitin-dependent protein catabolic process chromosome segregation mitotic nuclear division viral process protein sumoylation positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of transcription, DNA-templated cell division global genome nucleotide-excision repair positive regulation of SUMO transferase activity
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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