Invitrogen

Transferrin Receptor Polyclonal Antibody

Name: Transferrin Receptor Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95212

View all (135) Transferrin Receptor (CD71) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

Transferrin Receptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Transferrin Receptor Antibody (PA5-95212) in ICC/IF

Immunocytochemistry analysis of TFRC using anti-TFRC antibody (Product # PA5-95212). TFRC was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-TFRC antibody (Product # PA5-95212) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used... View More

Transferrin Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Transferrin Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Transferrin Receptor Polyclonal Antibody

Product Details

PA5-95212

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human TFRC recombinant protein (Position: M1-N198).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2807016

Product Specific Information

Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human K562 whole cell, human HT1080 whole cell, rat thymus tissue, rat RAW2647 whole cell, mouse SP2/0 whole cell. IHC: human placenta tissue IHC-F: human placenta tissue. ICC/IF: A431 cell. Flow: SiHa cell, U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD antigen CD71; CD71; mammary tumor virus receptor 1; p90; T9; tfR; tfR1; TR; Transferrin receptor protein 1

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Gene Aliases: 2610028K12Rik; AI195355; AI426448; AU015758; CD71; E430033M20Rik; IMD46; Mtvr-1; Mtvr1; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786, (Rat) Q99376, (Mouse) Q62351

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Entrez Gene ID: (Human) 7037, (Rat) 64678, (Mouse) 22042

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Function(s)

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding iron ion transmembrane transporter activity

Process(es)

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import receptor-mediated endocytosis response to organic substance response to inorganic substance endocytosis iron ion transmembrane transport protein transmembrane transport

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Transferrin Receptor Polyclonal Antibody

Transferrin Receptor Antibody (PA5-95212) in ICC/IF

Product Details

PA5-95212

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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