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  • Transferrin Antibodies

Invitrogen

Transferrin Monoclonal Antibody (F2H8G6)

View all (97) Transferrin antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Transferrin Monoclonal Antibody (F2H8G6)

  • Antibody Testing Data (2)
Transferrin Antibody in ELISA (ELISA)
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Transferrin Antibody in ELISA (ELISA)
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Transferrin Antibody (MA5-14690) in ELISA

Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100 µL of an transferrin chicken polyclonal antibody (Product # PA1-9525) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382) overnight at 4C. Wells were blocked with 100 µL of StartingBlock T20 (TBS) Blocking Buffer (Product # 28382) for 2 hours, and 100 µL of recombinant transferrin protein ranging from 1.6-1000 ng/mL were incubated for 1 hour at room temperature. The plate was washed with 1X TBST (Product # 28360), an... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Transferrin Antibody in Immunocytochemistry (ICC/IF)
Transferrin Antibody in ELISA (ELISA)
Transferrin Monoclonal Antibody (F2H8G6)

Product Details

MA5-14690

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:100-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-

Radioimmune Assays (RIA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F2H8G6

Immunogen

Human Transferrin
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10983676

Product Specific Information

MA5-14690 has been successfully used in ELISA, immunofluorescence, and immunoprecipitation, and radioimmune assay applications, and reacts with human samples.

MA5-14690 detects Transferrin which has a predicted molecular weight of approximately 67 kDa.

Product MA514690 is a smaller package size of MIT0603 (formerly sold as a Seradyn product).

Target Information

Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta-1 metal-binding globulin; DKFZp781D0156; epididymis secretory sperm binding protein Li 71p; Serotransferrin; Siderophilin; transferin; Transferrin

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Gene Aliases: HEL-S-71p; PRO1400; PRO1557; PRO2086; TF; TFQTL1

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UniProt ID: (Human) P02787

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Entrez Gene ID: (Human) 7018

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Function(s)
protein binding ferrous iron binding ferric iron binding ferric iron transmembrane transporter activity transferrin receptor binding
Process(es)
retina homeostasis platelet degranulation cellular iron ion homeostasis regulation of protein stability transferrin transport positive regulation of receptor-mediated endocytosis iron ion homeostasis cellular response to iron ion ferrous iron import into cell
It has to be done as per old AB suggested Products section.
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