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  • Transferrin Antibodies

Rockland

Transferrin Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite Transferrin Polyclonal Antibody

  • Antibody Testing Data (2)
Transferrin Antibody in Western Blot (WB)
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Transferrin Antibody in Western Blot (WB)
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Transferrin Antibody (109-4134) in WB

Rockland Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Bio-Rad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in M... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Transferrin Antibody in Western Blot (WB)
Transferrin Antibody in Western Blot (WB)
Transferrin Polyclonal Antibody

Product Details

109-4134

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Transferrin [Human Serum]
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

82 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Transferrin [Human Serum]. Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined.

Target Information

Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta-1 metal-binding globulin; DKFZp781D0156; epididymis secretory sperm binding protein Li 71p; Serotransferrin; Siderophilin; transferin; Transferrin

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Gene Aliases: HEL-S-71p; PRO1400; PRO1557; PRO2086; TF; TFQTL1

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UniProt ID: (Human) P02787

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Entrez Gene ID: (Human) 7018

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Function(s)
protein binding ferrous iron binding ferric iron binding ferric iron transmembrane transporter activity transferrin receptor binding
Process(es)
retina homeostasis platelet degranulation cellular iron ion homeostasis regulation of protein stability transferrin transport positive regulation of receptor-mediated endocytosis iron ion homeostasis cellular response to iron ion ferrous iron import into cell
It has to be done as per old AB suggested Products section.
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