Invitrogen

Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (1O2H10)

Name: Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (1O2H10)
Brand: Invitrogen
SKU: MA5-38102

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View all (36) Thioredoxin 1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)
Advanced Verification (1)

Thioredoxin 1 Antibody in Immunocytochemistry (ICC/IF)

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Thioredoxin 1 Antibody (MA5-38102) in ICC/IF

Immunofluorescence analysis of TXN was performed using 70% confluent log phase HeLa cells treated with PMA (30 ng/mL) for 24hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (ARC0885) (Product # MA5... View More

Thioredoxin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Thioredoxin 1 Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (1O2H10)

Product Details

MA5-38102

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:8,000

Immunocytochemistry (ICC/IF)

1:100

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1O2H10

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 26-105 of human Thioredoxin 1 (Trx1/TXN) (P10599)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2898020

Product Specific Information

Positive Samples: HeLa, HepG2, MCF7

Immunogen sequence: DFSATWCGPC KMIKPFFHSL SEKYSNVIFL EVDVDDCQDV ASECEVKCMP TFQFFKKGQK VGEFSGANKE KLEATINELV

Target Information

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADF; ATL-derived factor; DKFZp686B1993; MGC61975; RP11-427L11.1; SASP; Surface-associated sulphydryl protein; testicular tissue protein Li 199; Thioredoxin; thioredoxin delta 3; Trx; TXN delta 3

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Gene Aliases: ADF; AW550880; TRDX; TRX; TRX1; TXN; Txn1

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UniProt ID: (Human) P10599, (Rat) P11232, (Mouse) P10639

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Entrez Gene ID: (Human) 7295, (Rat) 116484, (Mouse) 22166

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Function(s)

protein binding protein disulfide oxidoreductase activity peptide disulfide oxidoreductase activity oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor poly(A) RNA binding thioredoxin-disulfide reductase activity enzyme binding

Process(es)

sulfate assimilation negative regulation of transcription from RNA polymerase II promoter response to reactive oxygen species transcription, DNA-templated protein folding glycerol ether metabolic process movement of cell or subcellular component signal transduction cell-cell signaling cell proliferation response to radiation nucleobase-containing small molecule interconversion protein repair activation of protein kinase B activity positive regulation of peptidyl-serine phosphorylation regulation of protein import into nucleus, translocation positive regulation of DNA binding cell redox homeostasis negative regulation of protein export from nucleus positive regulation of protein kinase B signaling oxidation-reduction process negative regulation of hydrogen peroxide-induced cell death regulation of transcription, DNA-templated response to selenium ion response to activity cellular response to drug response to drug response to axon injury cellular response to antibiotic cellular response to glucose stimulus cellular response to hyperoxia response to dexamethasone response to thyroxine transport cellular response to oxidative stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (1O2H10)

Thioredoxin 1 Antibody (MA5-38102) in ICC/IF

Product Details

MA5-38102

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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