AbboMax

Telomerase Polyclonal Antibody

Name: Telomerase Polyclonal Antibody
Brand: AbboMax
SKU: 500-11034

View all (37) TERT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Telomerase Antibody in Western Blot (WB)

FIGURE: 1 / 1

Telomerase Antibody (500-11034) in WB

Western Blot: 5 mg of the total protein derived from the cell lysate of 3T3 (left lane) and MCF-7 (right lane) were immunoprecipitated by 5 µg of the Rabbit anti Telomerase (Cat#500-11034), extract and separated onto 7.5% SDS-PAGE, transferred onto NC membrane and followed by immunoblotting of the same primary antibody at 1:1000. An immunoreactive bands was observed around ~127 kDa. (50 kDa and 25 kDa are light and heavy chains respectively.

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Immunogen image

Immunogen Image Telomerase Polyclonal Antibody

Product Details

500-11034

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the C-term of TERT protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive control: Breast Cancer

Cellular location: Nuclear.

Target Information

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; TP2

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Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT

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UniProt ID: (Human) O14746

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Entrez Gene ID: (Human) 7015

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Function(s)

tRNA binding transcription coactivator binding DNA binding telomerase activity telomerase RNA reverse transcriptase activity RNA binding RNA-directed DNA polymerase activity RNA-directed RNA polymerase activity protein binding nucleotidyltransferase activity telomeric DNA binding protein homodimerization activity metal ion binding telomerase RNA binding

Process(es)

telomere maintenance transcription, RNA-templated RNA-dependent DNA biosynthetic process telomere maintenance via telomerase mitochondrion organization negative regulation of gene expression DNA strand elongation positive regulation of Wnt signaling pathway production of siRNA involved in RNA interference regulation of protein stability positive regulation of protein binding RNA biosynthetic process positive regulation of hair cycle positive regulation of nitric-oxide synthase activity establishment of protein localization to telomere cellular response to hypoxia DNA biosynthetic process replicative senescence positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of siRNA involved in RNA interference positive regulation of protein localization to nucleolus beta-catenin-TCF complex assembly positive regulation of stem cell proliferation negative regulation of cellular senescence negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Telomerase Polyclonal Antibody

Telomerase Antibody (500-11034) in WB

Product Details

500-11034

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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