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  • TUBA1A Antibodies

Invitrogen

TUBA1A Polyclonal Antibody

9 Published Figures
13 References
View all (7) TUBA1A antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TUBA1A Polyclonal Antibody

  • Antibody Testing Data (12)
  • Published Figures (9)
TUBA1A Antibody in Immunocytochemistry (ICC/IF)
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TUBA1A Antibody in Immunocytochemistry (ICC/IF)
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TUBA1A Antibody (PA5-29444) in ICC/IF

Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-29544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 co... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TUBA1A Antibody in Immunocytochemistry (ICC/IF)
TUBA1A Antibody in Immunocytochemistry (ICC/IF)
TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)
TUBA1A Antibody in Western Blot (WB)

Product Details

PA5-29444

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:20,000
View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 2 publications 2 publications
Product Specifications

Species Reactivity

Chicken, Fruit fly, Human, Insect, Mouse, Non-human primate, Protozoa, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris/0.1M glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546920

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, HeLa cytosol fraction, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2, Drosophila.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 1a antibody; alpha 3 antibody; Alpha-tubulin 1; Alpha-tubulin 3; alpha-tubulin 3 antibody; Alpha-tubulin isotype M-alpha-1; B-ALPHA-1 antibody; brain-specific antibody; FLJ25113 antibody; FLJ30169; H2 ALPHA; H2-ALPHA; H2ALPHA; hum-a-tub1; hum-a-tub1 antibody; hum-a-tub2; hum-a-tub2 antibody; LIS3 antibody; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SW-cl.56; OK/SWcl.56; Talpha1 alpha-tubulin; TUBA1A antibody; TUBA3 antibody; TUBB1; TUBB5; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1A chain antibody; Tubulin alpha-3 chain; tubulin alpha-3 chain antibody; Tubulin B-alpha-1; tubulin B-alpha-1 antibody; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific

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Gene Aliases: B-ALPHA-1; LIS3; Tuba-1; TUBA1; TUBA1A; TUBA3; TUBA6

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UniProt ID: (Human) Q71U36, (Chicken) P02552, (Mouse) P68369, (Rat) P68370

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Entrez Gene ID: (Human) 7846, (Chicken) 429035, (Mouse) 22142, (Rat) 64158

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Function(s)
GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding nucleotide binding protein heterodimerization activity
Process(es)
microtubule-based process protein polymerization G2/M transition of mitotic cell cycle cytoskeleton organization cytoskeleton-dependent intracellular transport cell division metabolic process
It has to be done as per old AB suggested Products section.
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