Invitrogen

TSPO Recombinant Rabbit Monoclonal Antibody (23G2)

Name: TSPO Recombinant Rabbit Monoclonal Antibody (23G2)
Brand: Invitrogen
SKU: MA5-33203

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View all (21) TSPO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)
Advanced Verification (3)

TSPO Antibody in Immunocytochemistry (ICC/IF)

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TSPO Antibody (MA5-33203) in ICC/IF

Immunofluorescence analysis of TSPO was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (Product # MA5-33203) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluo... View More

TSPO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TSPO Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of TSPO was observed in THP1, Raw 264.7, HeLa, MCF7, Caki 1, PC3 in comparison to SK-O-V3 and HepG2 using Anti-TSPO Recombinant Rabbit Monoclonal Antibody (Product # MA5-33203) in Western Blot. {RE}

Immunogen image

Immunogen Image TSPO Recombinant Rabbit Monoclonal Antibody (23G2)

Product Details

MA5-33203

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

1:200-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

23G2

Immunogen

A synthesized peptide derived from human TSPO (50-169aa)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.94 mg/mL

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2812019

Target Information

Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: benzodiazepine peripheral binding site; benzodiazepine receptor, peripheral; isoquinoline-binding protein; Mitochondrial benzodiazepine receptor; OTTHUMP00000028891; PBR; Peripheral-type benzodiazepine receptor; PKBS; Translocator protein; translocator protein (18kDa)

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Gene Aliases: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR; TSPO; Tspo1

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UniProt ID: (Human) P30536, (Mouse) P50637

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Entrez Gene ID: (Human) 706, (Mouse) 12257

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Function(s)

androgen binding protein binding benzodiazepine receptor activity cholesterol binding ion channel binding

Process(es)

steroid biosynthetic process transport ion transport chloride transport lipid transport signal transduction chemical synaptic transmission glial cell migration positive regulation of necrotic cell death negative regulation of tumor necrosis factor production positive regulation of apoptotic process negative regulation of nitric oxide biosynthetic process behavioral response to pain regulation of steroid biosynthetic process positive regulation of mitochondrial depolarization positive regulation of calcium ion transport contact inhibition positive regulation of glial cell proliferation negative regulation of glial cell proliferation positive regulation of reactive oxygen species metabolic process protein targeting to mitochondrion heme biosynthetic process anion transport apoptotic process aging steroid metabolic process cell proliferation response to manganese ion response to vitamin B1 peripheral nervous system axon regeneration adrenal gland development negative regulation of protein ubiquitination regulation of cholesterol transport response to progesterone response to testosterone response to drug cellular response to lipopolysaccharide cellular response to zinc ion cellular hypotonic response maintenance of protein location in mitochondrion negative regulation of mitophagy negative regulation of ATP metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TSPO Recombinant Rabbit Monoclonal Antibody (23G2)

TSPO Antibody (MA5-33203) in ICC/IF

Product Details

MA5-33203

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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