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  • Primary Antibodies ›
  • TREM1 Antibodies

Invitrogen

TREM1 Polyclonal Antibody

2 Published Figures
1 Reference
View all (28) TREM1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TREM1 Polyclonal Antibody

  • Antibody Testing Data (3)
  • Published Figures (2)
TREM1 Antibody in Western Blot (WB)
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TREM1 Antibody in Western Blot (WB)
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TREM1 Antibody (PA5-95477) in WB

Western blot analysis of TREM1 in Lane 1: recombinant human TREM1 protein (1 ng). Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with TREM1 polyclonal antibody (Product # PA5-95477) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TREM1 Antibody in Western Blot (WB)
TREM1 Antibody in Flow Cytometry (Flow)
TREM1 Antibody in Flow Cytometry (Flow)
TREM1 Antibody in Western Blot (WB)
TREM1 Antibody in Western Blot (WB)
TREM1 Polyclonal Antibody

Product Details

PA5-95477

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human TREM1 recombinant protein (Position: A21-R200).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807279

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - Flow: Human PBMC cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

TREM1 (triggering receptor expressed on myeloid cells 1) is a receptor belonging to the Ig superfamily that amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. It is thought that TREM1 other related TREM proteins such as TREM2 act to modulate the inflammatory response. TREM1 has also been linked to inflammatory bowel disease resulting in enhanced production of TNF, IL-6, IL-8, and MCP-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD354; sCD354; soluble CD354; soluble TREM 1; soluble TREM1; sTREM 1; sTREM1; TREM; TREM-1; Triggering receptor expressed on monocytes 1; Triggering receptor expressed on myeloid cells; Triggering receptor expressed on myeloid cells 1

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Gene Aliases: CD354; TREM-1; TREM1

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UniProt ID: (Human) Q9NP99

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Entrez Gene ID: (Human) 54210

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Function(s)
receptor activity scaffold protein binding
Process(es)
positive regulation of defense response to virus by host cytokine secretion involved in immune response humoral immune response neutrophil chemotaxis intracellular signal transduction innate immune response chemokine metabolic process regulation of immune response leukocyte migration neutrophil mediated killing of gram-negative bacterium neutrophil extravasation mitophagy in response to mitochondrial depolarization xenophagy
It has to be done as per old AB suggested Products section.
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