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Invitrogen
TNFR2 (soluble) Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
TNFR2 (soluble) Antibody (500-P168-1MG) in ELISA
Product Details
500-P168-1MG
Species Reactivity
Published species
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Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type II matrix.
Sandwich ELISA: To detect Human sTNF Receptor Type II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type II (500-P168Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type II.
Western Blot: To detect Human sTNF Receptor Type II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD120b; p75; p75 TNF receptor; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF-R2; TNF-RII; TNFR; TNFR II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Gene Aliases: CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; TNFRSF1B
UniProt ID: (Human) P20333
Entrez Gene ID: (Human) 7133
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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