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  • TNF alpha Antibodies

Invitrogen

TNF alpha Recombinant Rabbit Monoclonal Antibody (3H15L1)

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View all (207) TNF alpha antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TNF alpha Recombinant Rabbit Monoclonal Antibody (3H15L1)

  • Antibody Testing Data (1)
TNF alpha Antibody in ELISA (ELISA)
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TNF alpha Antibody in ELISA (ELISA)
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TNF alpha Antibody (701264) in ELISA

Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a rabbit anti-TNF alpha monoclonal antibody (Product # M302) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minute... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TNF alpha Antibody in ELISA (ELISA)
TNF alpha Recombinant Rabbit Monoclonal Antibody (3H15L1)

Product Details

701264

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

ELISA (ELISA)

1:25-1:3200
-
Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H15L1

Immunogen

Recombinant protein corresponding to amino acids 77-233 of human TNF-alpha
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532447

Product Specific Information

P300A was successfully used to detect human TNF alpha in ELISA application.

This antibody is predicted to react with mouse, rat and non-human primate based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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Function(s)
protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity positive regulation of cytokine production positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response humoral immune response I-kappaB kinase/NF-kappaB signaling JNK cascade extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus response to salt stress positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity regulation of tumor necrosis factor-mediated signaling pathway negative regulation of lipid storage extracellular matrix organization osteoclast differentiation sequestering of triglyceride cortical actin cytoskeleton organization positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion negative regulation of myosin-light-chain-phosphatase activity positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import embryonic digestive tract development positive regulation of smooth muscle cell proliferation positive regulation of cytokine secretion positive regulation of phagocytosis regulation of insulin secretion defense response to Gram-positive bacterium leukocyte tethering or rolling negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound death-inducing signaling complex assembly positive regulation of mononuclear cell migration positive regulation of podosome assembly establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of NIK/NF-kappaB signaling positive regulation of superoxide dismutase activity regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell positive regulation of protein localization to cell surface positive regulation of ceramide biosynthetic process positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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