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  • TNF alpha Antibodies

Invitrogen

TNF alpha Polyclonal Antibody, PeproTech®

5 References
View all (207) TNF alpha antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TNF alpha Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
TNF alpha Antibody in ELISA (ELISA)
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TNF alpha Antibody in ELISA (ELISA)
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TNF alpha Antibody (500-P64G-1MG) in ELISA

Sandwich ELISA: To detect Murine TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TNF alpha Polyclonal Antibody (Product # 500-P64G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TNF alpha Polyclonal Antibody, Biotin (Product # 500-P64GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine TNF-alpha. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TNF alpha Antibody in Western Blot (WB)
TNF alpha Antibody in Western Blot (WB)
TNF alpha Antibody in ELISA (ELISA)
TNF alpha Polyclonal Antibody, PeproTech®

Product Details

500-P64G-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
View 3 publications 3 publications

In vitro Assay (IV)

-
View 1 publication 1 publication

Immunostaining (IS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Bacteria, Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine TNF-alpha
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930678

Product Specific Information

AA Sequence of recombinant protein: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine TNF-alpha. Anti-Murine TNF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Murine TNF-alpha matrix.

Sandwich ELISA: To detect Murine TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine TNF-alpha (500-P64GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine TNF-alpha.

Western Blot: To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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Function(s)
protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity cell activation positive regulation of cytokine production regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of L-glutamate transport chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process obsolete transformed cell apoptotic process defense response inflammatory response immune response humoral immune response signal transduction JNK cascade multicellular organism development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus organ morphogenesis positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation sequestering of triglyceride positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-18 production positive regulation of interleukin-6 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion regulation of cell proliferation positive regulation of NF-kappaB import into nucleus defense response to bacterium positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process cellular extravasation positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation regulation of protein secretion positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of phagocytosis regulation of insulin secretion positive regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound positive regulation of podosome assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of protein localization to cell surface positive regulation of chemokine (C-X-C motif) ligand 2 production regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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