Bethyl Laboratories

TIF1 Alpha/TRIM24 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
TIF1 Alpha/TRIM24 Polyclonal Antibody

Product Details

A300-815A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-

ChIP-sequencing (ChIP-Seq)

4 µg/30 µg chromatin
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 700 and 750 of Human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; RING-type E3 ubiquitin transferase TIF1-alpha; TIF1-alpha; Transcription intermediary factor 1-alpha; transcriptional intermediary factor 1; transcriptional intermediary factor 1, alpha; tripartite motif-containing 24; Tripartite motif-containing protein 24

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Gene Aliases: A130082H20Rik; AI447469; D430004I05Rik; hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1-alpha; TIF1A; TIF1ALPHA; TRIM24

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UniProt ID: (Human) O15164, (Mouse) Q64127

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Entrez Gene ID: (Human) 8805, (Mouse) 21848

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Function(s)
p53 binding chromatin binding transcription coactivator activity protein tyrosine kinase activity ubiquitin-protein transferase activity receptor binding protein binding zinc ion binding ligase activity ligand-dependent nuclear receptor binding estrogen response element binding methylated histone binding sequence-specific DNA binding ubiquitin protein ligase activity lysine-acetylated histone binding DNA binding protein kinase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
transcription from RNA polymerase II promoter negative regulation of cell proliferation protein ubiquitination peptidyl-tyrosine phosphorylation protein catabolic process regulation of protein stability regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated protein autophosphorylation calcium ion homeostasis regulation of vitamin D receptor signaling pathway cellular response to estrogen stimulus regulation of signal transduction by p53 class mediator transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation positive regulation of gene expression regulation of nucleic acid-templated transcription
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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