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Invitrogen
TGF alpha Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 1
TGF alpha Antibody (500-P16BT-25UG) in ELISA
Product Details
500-P16BT-25UG
Species Reactivity
Host/Isotype
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Type
Immunogen
Conjugate
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Purification
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RRID
Product Specific Information
AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TGF-alpha (500-P16) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha.
Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P16BT-1MG will be provided as 2 x 500 µg
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: Protransforming growth factor alpha; TGF a; TGF α; TGF-alpha; TGFalpha; TGFα; Transforming growth factor; transforming growth factor, alpha
Gene Aliases: TFGA; TGFA
UniProt ID: (Human) P01135
Entrez Gene ID: (Human) 7039
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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