Invitrogen

TERT Monoclonal Antibody (2D8)

Name: TERT Monoclonal Antibody (2D8)
Brand: Invitrogen
SKU: MA5-16033

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2 References

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

TERT Antibody in Immunocytochemistry (ICC/IF)

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TERT Antibody (MA5-16033) in ICC/IF

Immunofluorescence analysis of TERT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Anti-TERT Mouse Monoclonal Antibody (2D8) (Product # MA5-16033) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More

TERT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TERT Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TERT siRNA and decrease in signal was observed in ICC using TERT Monoclonal Antibody (2D8) (Product # MA5-16033). {KD}

Immunogen image

Immunogen Image TERT Monoclonal Antibody (2D8)

Product Details

MA5-16033

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:200

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2D8

Immunogen

Full-length recombinant human Telomerase reverse transcriptase from insect cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153520

Product Specific Information

Suggested positive control: 293T whole cell lysates, HeLa whole cell lysates, antigen standard for TERT (transient overexpression lysate).

Target Information

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; TP2

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Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT

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UniProt ID: (Human) O14746

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Entrez Gene ID: (Human) 7015

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Function(s)

tRNA binding transcription coactivator binding DNA binding telomerase activity telomerase RNA reverse transcriptase activity RNA binding RNA-directed DNA polymerase activity RNA-directed RNA polymerase activity protein binding nucleotidyltransferase activity telomeric DNA binding protein homodimerization activity metal ion binding telomerase RNA binding

Process(es)

telomere maintenance transcription, RNA-templated RNA-dependent DNA biosynthetic process telomere maintenance via telomerase mitochondrion organization negative regulation of gene expression DNA strand elongation positive regulation of Wnt signaling pathway production of siRNA involved in RNA interference regulation of protein stability positive regulation of protein binding RNA biosynthetic process positive regulation of hair cycle positive regulation of nitric-oxide synthase activity establishment of protein localization to telomere cellular response to hypoxia DNA biosynthetic process replicative senescence positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of siRNA involved in RNA interference positive regulation of protein localization to nucleolus beta-catenin-TCF complex assembly positive regulation of stem cell proliferation negative regulation of cellular senescence negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TERT Monoclonal Antibody (2D8)

TERT Antibody (MA5-16033) in ICC/IF

Product Details

MA5-16033

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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