Invitrogen

TERT Polyclonal Antibody

Name: TERT Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-142844

View all (37) TERT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

TERT Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

TERT Antibody (PA5-142844) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of TERT in HeLa cells using TERT Polyclonal Antibody (Product # PA5-142844). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2... View More

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Immunogen Image TERT Polyclonal Antibody

Product Details

PA5-142844

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence C-QLRELSEAEVRQHRE.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2933487

Product Specific Information

Additional Information: This antibody is expected to recognize both reported isoforms (NP_937983.2; (NP_001180305.1).

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

Target Information

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; TP2

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Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT

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UniProt ID: (Human) O14746

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Entrez Gene ID: (Human) 7015

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Function(s)

tRNA binding transcription coactivator binding DNA binding telomerase activity telomerase RNA reverse transcriptase activity RNA binding RNA-directed DNA polymerase activity RNA-directed RNA polymerase activity protein binding nucleotidyltransferase activity telomeric DNA binding protein homodimerization activity metal ion binding telomerase RNA binding

Process(es)

telomere maintenance transcription, RNA-templated RNA-dependent DNA biosynthetic process telomere maintenance via telomerase mitochondrion organization negative regulation of gene expression DNA strand elongation positive regulation of Wnt signaling pathway production of siRNA involved in RNA interference regulation of protein stability positive regulation of protein binding RNA biosynthetic process positive regulation of hair cycle positive regulation of nitric-oxide synthase activity establishment of protein localization to telomere cellular response to hypoxia DNA biosynthetic process replicative senescence positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of siRNA involved in RNA interference positive regulation of protein localization to nucleolus beta-catenin-TCF complex assembly positive regulation of stem cell proliferation negative regulation of cellular senescence negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

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TERT Polyclonal Antibody

TERT Antibody (PA5-142844) in ICC/IF

Product Details

PA5-142844

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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