Invitrogen

TAB1 Monoclonal Antibody (F.211.6)

Name: TAB1 Monoclonal Antibody (F.211.6)
Brand: Invitrogen
SKU: MA5-14964

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View all (22) TAB1 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

FIGURE: 1 / 4

TAB1 Antibody (MA5-14964) in WB

Western blot was performed using TAB1 Monoclonal Antibody (Product # MA5-14964) and a 55 kDa band corresponding to TAB1 was observed across all the cell lines and tissues tested. An uncharacterized band (*) at~45 kDa was also observed. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), SKOV-3 (Lane 5), PC-12 (Lane 6), HepG2 (Lane 7), tissue extracts of Mouse Brain (Lane 8), Mouse Lung (Lane 9), Mouse Liver (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE™ 10%... View More

Product Image Gallery

TAB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TAB1 siRNA and decrease in signal intensity was observed in western blot application using TAB1 Monoclonal Antibody (Product # MA5-14964). {KD}

Immunogen image

Immunogen Image TAB1 Monoclonal Antibody (F.211.6)

Product Details

MA5-14964

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunoprecipitation (IP)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.211.6

Immunogen

Synthetic peptide corresponding to residues surrounding Pro381 of human TAB1

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

25 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10977539

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 (TAB1) is a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. TAB1 interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. It can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta activated kinase-1 binding protein-1; MGC57664; mitogen activated protein kinase kinase kinase 7 interacting protein 1; mitogen-activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; RP3-407F17.2; TAB1beta; TAK1-binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1

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Gene Aliases: 2310012M03Rik; 3'-Tab1; b2b449Clo; MAP3K7IP1; TAB1

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UniProt ID: (Human) Q15750, (Mouse) Q8CF89

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Entrez Gene ID: (Human) 10454, (Mouse) 66513, (Rat) 315139

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Function(s)

protein serine/threonine phosphatase activity protein binding enzyme activator activity kinase activator activity catalytic activity protein complex binding mitogen-activated protein kinase p38 binding

Process(es)

activation of MAPKKK activity in utero embryonic development heart morphogenesis transforming growth factor beta receptor signaling pathway lung development positive regulation of MAP kinase activity activation of MAPK activity stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway cardiac septum development protein dephosphorylation I-kappaB kinase/NF-kappaB signaling JNK cascade aorta development Fc-epsilon receptor signaling pathway positive regulation of NF-kappaB transcription factor activity coronary vasculature development nucleotide-binding oligomerization domain containing signaling pathway metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TAB1 Monoclonal Antibody (F.211.6)

TAB1 Antibody (MA5-14964) in WB

Product Details

MA5-14964

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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