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  • Syndecan 4 Antibodies

Invitrogen

Syndecan 4 Recombinant Rabbit Monoclonal Antibody (008)

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Datasheet
Protocols
Questions & Answers

Cite Syndecan 4 Recombinant Rabbit Monoclonal Antibody (008)

  • Antibody Testing Data (1)
Syndecan 4 Antibody in Immunocytochemistry (ICC/IF)
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Syndecan 4 Antibody in Immunocytochemistry (ICC/IF)
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Syndecan 4 Antibody (MA5-31167) in ICC/IF

Immunofluorescence staining of mSyndecan 4 in mouse spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Syndecan 4 Recombinant Rabbit Monoclonal Antibody (8) (Product # MA5-31167, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Syndecan 4 Antibody in Immunocytochemistry (ICC/IF)
Syndecan 4 Recombinant Rabbit Monoclonal Antibody (008)

Product Details

MA5-31167

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:20-1:100
-

ELISA (ELISA)

1:5,000-1:10,000
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

008

Immunogen

Recombinant Mouse SDC4/Syndecan-4 protein (Met1-Val146)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2786817

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Mouse SDC4/Syndecan-4.

Target Information

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan 1 is a matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan 1 and Syndecan 3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan 2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan 4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC22217; Ryudocan core protein; SYND4; Syndecan-4

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Gene Aliases: AA959608; AW108331; ryudocan; S4; Sdc4; Synd4; syndecan-4

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UniProt ID: (Mouse) O35988

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Entrez Gene ID: (Mouse) 20971

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Function(s)
fibronectin binding protein kinase C binding thrombospondin receptor activity
Process(es)
neural tube closure signal transduction regulation of fibroblast migration cell migration wound healing positive regulation of protein kinase activity positive regulation of stress fiber assembly positive regulation of focal adhesion assembly inner ear receptor stereocilium organization positive regulation of exosomal secretion positive regulation of extracellular exosome assembly
It has to be done as per old AB suggested Products section.
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