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Sirtuin 3 (SIRT3) Monoclonal Antibody (PCRP-SIRT3-1C10)

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Datasheet
Protocols
Questions & Answers

Cite Sirtuin 3 (SIRT3) Monoclonal Antibody (PCRP-SIRT3-1C10)

  • Antibody Testing Data (4)
Sirtuin 3 (SIRT3) Antibody in Immunocytochemistry (ICC/IF)
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Sirtuin 3 (SIRT3) Antibody in Immunocytochemistry (ICC/IF)
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Sirtuin 3 (SIRT3) Antibody (23410-MSM1-P1ABX) in ICC/IF

Immunofluorescence analysis of PFA-fixed HeLa cells stained using SIRT3 Mouse Monoclonal Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Sirtuin 3 (SIRT3) Antibody in Immunocytochemistry (ICC/IF)
Sirtuin 3 (SIRT3) Antibody in Flow Cytometry (Flow)
Sirtuin 3 (SIRT3) Antibody in Peptide array (ARRAY)
Sirtuin 3 (SIRT3) Antibody in SDS-PAGE (SDS-PAGE)
Sirtuin 3 (SIRT3) Monoclonal Antibody (PCRP-SIRT3-1C10)

Product Details

23410-MSM1-P1ABX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

1-2 µg/100-500 µg total protein
-

Peptide Array (Array)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PCRP-SIRT3-1C10

Immunogen

Recombinant full-length human SIRT3 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; sirtuin type 3

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Gene Aliases: SIR2L3; SIRT3

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UniProt ID: (Human) Q9NTG7

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Entrez Gene ID: (Human) 23410

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Function(s)
NAD+ ADP-ribosyltransferase activity protein binding zinc ion binding NAD-dependent histone deacetylase activity (H3-K14 specific) NAD+ binding
Process(es)
protein ADP-ribosylation protein deacetylation mitochondrion organization aerobic respiration peptidyl-lysine deacetylation histone H3 deacetylation negative regulation of peptidyl-lysine acetylation
It has to be done as per old AB suggested Products section.
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