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  • CD75 Antibodies

Invitrogen

ST6GAL1 Polyclonal Antibody

View all (22) CD75 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ST6GAL1 Polyclonal Antibody

  • Antibody Testing Data (2)
ST6GAL1 Antibody in Western Blot (WB)
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ST6GAL1 Antibody in Western Blot (WB)
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ST6GAL1 Antibody (PA5-81043) in WB

Western Blot using ST6GAL1 Polyclonal Antibody (Product # PA5-81043) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 55 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ST6GAL1 Antibody in Western Blot (WB)
ST6GAL1 Antibody in Immunoprecipitation (IP)

Product Details

PA5-81043

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

4-6 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human ST6GAL1/ST6GAL-I protein (Lys27-Cys406)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2788295

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human ST6GAL1/ST6GAL-I.

Target Information

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alpha 2,6-ST 1; B-cell antigen CD75; Beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; Sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI

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Gene Aliases: SIAT1; ST6GAL1; ST6GalI; ST6N

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UniProt ID: (Human) P15907

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Entrez Gene ID: (Human) 6480

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Function(s)
beta-galactoside alpha-2,6-sialyltransferase activity protein binding sialyltransferase activity
Process(es)
N-acetylneuraminate metabolic process humoral immune response oligosaccharide metabolic process O-glycan processing protein N-linked glycosylation via asparagine positive regulation of mononuclear cell proliferation response to ethanol negative regulation of chemotaxis sialylation regulation of substrate adhesion-dependent cell spreading protein sialylation negative regulation of macrophage apoptotic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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