Invitrogen

SMAD6 Polyclonal Antibody

Name: SMAD6 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-25263

View all (17) SMAD6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

SMAD6 Antibody (PA5-25263) in WB

Western blot analysis of SMAD6 in Human lung whole cell lysate. Samples were incubated with SMAD6 polyclonal antibody (Product # PA5-25263) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Product Image Gallery

Immunogen image

Immunogen Image SMAD6 Polyclonal Antibody

Product Details

PA5-25263

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 358-386 amino acids from the central region of human SMAD6

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542763

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: MAD homolog 6; Mothers against decapentaplegic homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6; SMAD; SMAD 6; SMAD family member 6; SMAD SMAD; SMAD, mothers against DPP homolog 6

View more View less

Gene Aliases: AOVD2; HsT17432; MADH6; MADH7; SMAD6

View more View less

UniProt ID: (Human) O43541

View more View less

Entrez Gene ID: (Human) 4091

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity ubiquitin protein ligase binding type I transforming growth factor beta receptor binding identical protein binding transcription regulatory region DNA binding metal ion binding co-SMAD binding I-SMAD binding R-SMAD binding type I activin receptor binding

Process(es)

ureteric bud development outflow tract septum morphogenesis mitral valve morphogenesis pulmonary valve morphogenesis ventricular septum development transcription, DNA-templated immune response transforming growth factor beta receptor signaling pathway zygotic specification of dorsal/ventral axis negative regulation of cell proliferation negative regulation of SMAD protein complex assembly negative regulation of ossification BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway cell-substrate adhesion response to laminar fluid shear stress aorta development negative regulation of apoptotic process response to estrogen fat cell differentiation negative regulation of pathway-restricted SMAD protein phosphorylation coronary vasculature development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SMAD6 Polyclonal Antibody

SMAD6 Antibody (PA5-25263) in WB

Product Details

PA5-25263

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!