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  • SMAD5 Antibodies

Invitrogen

SMAD5 Monoclonal Antibody (3H9)

1 Published Figure
1 Reference
View all (34) SMAD5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite SMAD5 Monoclonal Antibody (3H9)

  • Antibody Testing Data (4)
  • Published Figures (1)
SMAD5 Antibody in Immunocytochemistry (ICC/IF)
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SMAD5 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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SMAD5 Antibody (MA5-15793) in ICC/IF

Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antibody (Product # MA5-15793) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SMAD5 Antibody in Immunocytochemistry (ICC/IF)
SMAD5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SMAD5 Antibody in Western Blot (WB)
SMAD5 Antibody in Flow Cytometry (Flow)
SMAD5 Antibody in Western Blot (WB)

Product Details

MA5-15793

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3H9

Immunogen

Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154325

Product Specific Information

MA5-15793 targets SMAD5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and Rat samples.

The MA5-15793 immunogen is purified recombinant fragment of human SMAD5 expressed in E. Coli.

MA5-15793 detects SMAD5 which has a predicted molecular weight of approximately 52kDa.

Target Information

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp781C1895; DKFZp781O1323; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5

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Gene Aliases: DWFC; JV5-1; MADH5; SMAD5

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UniProt ID: (Human) Q99717, (Rat) Q9R1V3

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Entrez Gene ID: (Human) 4090, (Rat) 59328

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Function(s)
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding metal ion binding
Process(es)
ureteric bud development Mullerian duct regression osteoblast fate commitment transcription, DNA-templated protein phosphorylation signal transduction transforming growth factor beta receptor signaling pathway germ cell development embryonic pattern specification erythrocyte differentiation BMP signaling pathway positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated cartilage development cardiac muscle contraction bone development SMAD protein signal transduction cellular response to organic cyclic compound positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus ossification regulation of ossification positive regulation of transcription from RNA polymerase II promoter cellular response to BMP stimulus
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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