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Invitrogen
SCF Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
SCF Antibody (500-P71BT-25UG) in ELISA
Product Details
500-P71BT-25UG
Species Reactivity
Host/Isotype
Class
Type
Immunogen
Conjugate
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Purification
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RRID
Product Specific Information
AA Sequence of recombinant protein: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SCF. Anti-Murine SCF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mSCF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SCF (500-P71) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mSCF.
Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P71BT-1MG will be provided as 2 x 500 µg
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: C-kit ligand; cloud gray; DKFZp686F2250; grizzle-belly; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Steel factor; Stem cell factor
Gene Aliases: blz; Clo; Con; contrasted; Gb; Kitl; Kitlg; Mgf; SCF; SF; Sl; SLF
UniProt ID: (Mouse) P20826
Entrez Gene ID: (Mouse) 17311
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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