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Invitrogen
Resistin Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
Resistin Antibody (500-P182G-1MG) in ELISA
Product Details
500-P182G-1MG
Species Reactivity
Host/Isotype
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Type
Immunogen
Conjugate
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RRID
Product Specific Information
AA Sequence of recombinant protein: SSMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVAS.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine Resistin. Anti-Murine Resistin-specific antibody was purified by affinity chromatography employing an immobilized Murine Resistin matrix.
Sandwich ELISA: To detect Murine Resistin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine Resistin (500-P182GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Resistin.
Western Blot: To detect Murine Resistin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Resistin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; Adipose-specific cysteine-rich secreted protein A12-alpha; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; cysteine-r; Cysteine-rich secreted protein FIZZ3; dominant inhibitory adipocyte-specific secretory factor; dri; found in inflammatory zone 3; MGC126603; MGC126609; Resistin; RSN
Gene Aliases: ADSF; Fizz3; Retn; Rstn; Xcp4
UniProt ID: (Mouse) Q99P87
Entrez Gene ID: (Mouse) 57264
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