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  • Renin Antibodies

Invitrogen

Renin Polyclonal Antibody

View all (36) Renin antibodies

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Datasheet
Protocols
Questions & Answers

Cite Renin Polyclonal Antibody

  • Antibody Testing Data (1)
Renin Antibody in Western Blot (WB)
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Renin Antibody in Western Blot (WB)
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Renin Antibody (PA5-116642) in WB

Western blot analysis of Renin in extracts of various cell lines. Samples were incubated with Renin Polyclonal antibody (Product # PA5-116642) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Renin Antibody in Western Blot (WB)
Renin Polyclonal Antibody

Product Details

PA5-116642

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 250-406 of human Renin (NP_0005281)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901273

Product Specific Information

Positive Samples: HepG2, HT-29, SKOV3, Mouse kidney, Mouse skin

Immunogen sequence: QHYEGNFHYI NLIKTGVWQI QMKGVSVGSS TLLCEDGCLA LVDTGASYIS GSTSSIEKLM EALGAKKRLF DYVVKCNEGP TLPDISFHLG GKEYTLTSAD YVFQESYSSK KLCTLAIHAM DIPPPTGPTW ALGATFIRKF YTEFDRRNNR IGFALAR

Target Information

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: angiotensin-forming enzyme; Angiotensinogenase; aspartyl-protease; EC 3.4.23; EC 3.4.23.15; FLJ10761; Kidney renin; Renin; renin 1 structural; renin b; renin precursor, renal; Renin-1

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Gene Aliases: D19352; HNFJ2; RATRENAA; REN; Ren-1; Ren-A; Ren1; Ren1c; Ren1d; RENAA; Rn-1; Rnr

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UniProt ID: (Human) P00797, (Rat) P08424, (Mouse) P06281

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Entrez Gene ID: (Human) 5972, (Rat) 24715, (Mouse) 19701

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Function(s)
aspartic-type endopeptidase activity receptor binding insulin-like growth factor receptor binding peptidase activity endopeptidase activity hydrolase activity
Process(es)
kidney development angiotensin maturation regulation of blood volume by renin-angiotensin renin-angiotensin regulation of aldosterone production proteolysis establishment of blood-nerve barrier regulation of blood pressure hormone-mediated signaling pathway response to organic substance response to drug drinking behavior regulation of MAPK cascade cell maturation response to cAMP response to cGMP mesonephros development male gonad development protein catabolic process response to lipopolysaccharide cellular response to drug response to immobilization stress beta-amyloid metabolic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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