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  • RPL5 Antibodies

Invitrogen

RPL5 Polyclonal Antibody

Advanced Verification
1 Reference
View all (9) RPL5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite RPL5 Polyclonal Antibody

  • Antibody Testing Data (8)
  • Advanced Verification (1)
RPL5 Antibody in Immunocytochemistry (ICC/IF)
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RPL5 Antibody in Immunocytochemistry (ICC/IF)
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RPL5 Antibody (PA5-27539) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of RPL5 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 Polyclonal Antibody (Product # PA5-27539) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RPL5 Antibody in Immunocytochemistry (ICC/IF)
RPL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RPL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RPL5 Antibody in Western Blot (WB)
RPL5 Antibody in Western Blot (WB)
RPL5 Antibody in Western Blot (WB)
RPL5 Antibody in Western Blot (WB)
RPL5 Antibody in Western Blot (WB)
RPL5 Antibody

Product Details

PA5-27539

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

-
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

in situ PLA (PLA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2545015

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat2.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Pig (97%), Rabbit (98%), Chicken (90%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RPL5 encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 60S ribosomal protein L5; Large ribosomal subunit protein uL18; protein phosphatase 1, regulatory subunit 135

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Gene Aliases: DBA6; L5; MSTP030; PPP1R135; RPL5; U21RNA

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UniProt ID: (Human) P46777, (Rat) P09895, (Mouse) P47962

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Entrez Gene ID: (Human) 6125, (Rat) 81763, (Mouse) 100503670

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Function(s)
RNA binding structural constituent of ribosome protein binding 5S rRNA binding poly(A) RNA binding rRNA binding
Process(es)
ribosomal large subunit assembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane viral transcription ribosomal large subunit biogenesis positive regulation of phosphatase activity cellular response to inorganic substance
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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