Invitrogen

RBM3 Polyclonal Antibody

Name: RBM3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-115830

View all (12) RBM3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (12)

RBM3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

RBM3 Antibody (PA5-115830) in ICC/IF

Immunocytochemistry analysis of RBM3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115830) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

RBM3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image RBM3 Polyclonal Antibody

Product Details

PA5-115830

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RBM3(Accession P98179), corresponding to amino acid residues G123-N157.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

AB_2900464

Product Specific Information

Antibody detects endogenous levels of total RBM3.

Target Information

RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000025800; OTTHUMP00000025802; putative RNA-binding protein 3; RNA binding motif protein 3; RNA-binding motif protein 3; RNA-binding protein 3; RNPL

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Gene Aliases: 2600016C11Rik; IS1-RNPL; RBM3; RNPL

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UniProt ID: (Human) P98179, (Mouse) O89086, (Rat) Q925G0

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Entrez Gene ID: (Human) 5935, (Mouse) 19652, (Rat) 114488

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Function(s)

nucleotide binding RNA binding protein binding poly(A) RNA binding nucleic acid binding ribosomal large subunit binding

Process(es)

translation regulation of translation response to cold production of miRNAs involved in gene silencing by miRNA positive regulation of translation RNA processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RBM3 Polyclonal Antibody

RBM3 Antibody (PA5-115830) in ICC/IF

Product Details

PA5-115830

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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