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  • Primary Antibodies ›
  • RAPGEF1 Antibodies

OriGene

RAPGEF1 Monoclonal Antibody (OTI2C3), TrueMAB™

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Datasheet
Protocols
Questions & Answers

Cite RAPGEF1 Monoclonal Antibody (OTI2C3), TrueMAB™

  • Antibody Testing Data (10)
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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RAPGEF1 Antibody (TA506838) in IHC (P)

Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506838) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RAPGEF1 Antibody in Western Blot (WB)
RAPGEF1 Antibody in Western Blot (WB)

Product Details

TA506838

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400-1:4,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2C3

Immunogen

Full length human recombinant protein of human RAPGEF1 produced in HEK293T cell.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CRK SH3-binding GNRP; guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1

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Gene Aliases: 4932418O06Rik; C3G; C3G-1; C3G-2; GRF2; RAPGEF1

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UniProt ID: (Human) Q13905

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Entrez Gene ID: (Human) 2889, (Dog) 491286, (Rat) 63881, (Mouse) 107746

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Function(s)
protein binding Rap guanyl-nucleotide exchange factor activity SH3 domain binding guanyl-nucleotide exchange factor activity signal transducer activity SH3/SH2 adaptor activity
Process(es)
activation of MAPKK activity blood vessel development signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of neuron projection development single organismal cell-cell adhesion Rap protein signal transduction nerve growth factor signaling pathway positive regulation of GTPase activity negative regulation of Ras protein signal transduction platelet-derived growth factor receptor signaling pathway negative regulation of protein kinase B signaling establishment of endothelial barrier negative regulation of ERK1 and ERK2 cascade cellular response to cAMP negative regulation of canonical Wnt signaling pathway regulation of cell junction assembly cellular response to nerve growth factor stimulus negative regulation of neural precursor cell proliferation small GTPase mediated signal transduction regulation of small GTPase mediated signal transduction positive regulation of signal transduction
It has to be done as per old AB suggested Products section.
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