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  • RAP Antibodies

Invitrogen

RAP Recombinant Rabbit Monoclonal Antibody (4)

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Datasheet
Protocols
Questions & Answers

Cite RAP Recombinant Rabbit Monoclonal Antibody (4)

  • Antibody Testing Data (3)
RAP Antibody in Immunocytochemistry (ICC/IF)
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RAP Antibody in Immunocytochemistry (ICC/IF)
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RAP Antibody (MA5-29399) in ICC/IF

Immunofluorescence staining of Human RAP in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RAP Recombinant Rabbit Monoclonal Antibody (4) (Product # MA5-29399, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RAP Antibody in Immunocytochemistry (ICC/IF)
RAP Antibody in Western Blot (WB)
RAP Antibody in Immunoprecipitation (IP)
RAP Recombinant Rabbit Monoclonal Antibody (4)

Product Details

MA5-29399

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:20-1:100
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

1-4 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4

Immunogen

Recombinant Human LRPAP1 protein (Tyr35-Leu357)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785267

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human LRPAP1/A2MRAP.

Target Information

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 39 kDa receptor-associated protein; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; low density lipoprotein receptor-related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); RAP

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Gene Aliases: A2MRAP; A2RAP; alpha-2-MRAP; HBP44; LRPAP1; MRAP; MYP23; RAP

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UniProt ID: (Human) P30533

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Entrez Gene ID: (Human) 4043

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Function(s)
asialoglycoprotein receptor activity protein binding heparin binding lipase binding receptor antagonist activity low-density lipoprotein particle receptor binding unfolded protein binding very-low-density lipoprotein particle receptor binding
Process(es)
protein folding receptor-mediated endocytosis negative regulation of very-low-density lipoprotein particle clearance vesicle-mediated transport negative regulation of protein binding extracellular negative regulation of signal transduction negative regulation of beta-amyloid clearance
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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