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  • RAGE Antibodies

Invitrogen

RAGE Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (26) RAGE antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite RAGE Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (1)
  • Advanced Verification (1)
RAGE Antibody in Western Blot (WB)
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RAGE Antibody in Western Blot (WB)
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RAGE Antibody (PA5-48057) in WB

Western blot was performed using Anti-RAGE Polyclonal Antibody (Product # PA5-48057) and 45, 55 kDa bands corresponding to RAGE was observed in Mouse Lung, Rat Lung but not in Mouse Heart, Mouse Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Rat Lung (Lane 2), Mouse Heart (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RAGE Antibody in Western Blot (WB)
RAGE Antibody in Western Blot (WB)
RAGE Antibody

Product Details

PA5-48057

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-
Product Specifications

Species Reactivity

Dog

Published species

Rat

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine RAGE Asp25-Val339

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576242

Product Specific Information

In sandwich ELISAs, approximately 0.5% cross-reactivity with recombinant human RAGE is observed and less than 0.1% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: advanced glycation end product receptor; RAGE-4 ORF3; RAGE/AGER; sRAGE

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Gene Aliases: RAGE

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Entrez Gene ID: (Dog) 403168

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It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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