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  • Presenilin 1 Antibodies

Invitrogen

Presenilin 1 Polyclonal Antibody

View all (25) Presenilin 1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Presenilin 1 Polyclonal Antibody

  • Antibody Testing Data (5)
Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
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Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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Presenilin 1 Antibody (PA5-30585) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Presenilin 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Presenilin 1 Polyclonal Antibody (Product # PA5 30585) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)
Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Presenilin 1 Antibody in Western Blot (WB)

Product Details

PA5-30585

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2548059

Product Specific Information

Recommended positive controls: HeLa.

Predicted reactivity: Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alzheimer Disease 3; Minilin; Presenilin-1; presenilin1; Protein S182; PS-1; S182 protein; Senilin 1

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Gene Aliases: AD3; Ad3h; FAD; PS-1; PS1; PSEN1; PSNL1; S182

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UniProt ID: (Human) P49768, (Mouse) P49769

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Entrez Gene ID: (Human) 5663, (Mouse) 19164

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Function(s)
endopeptidase activity aspartic-type endopeptidase activity calcium channel activity protein binding beta-catenin binding PDZ domain binding cadherin binding peptidase activity hydrolase activity aspartic endopeptidase activity, intramembrane cleaving
Process(es)
autophagosome assembly negative regulation of transcription from RNA polymerase II promoter activation of MAPKK activity blood vessel development cell fate specification somitogenesis neuron migration positive regulation of receptor recycling heart looping hematopoietic progenitor cell differentiation T cell activation involved in immune response neural retina development protein glycosylation membrane protein ectodomain proteolysis mitochondrial transport cellular response to DNA damage stimulus response to oxidative stress negative regulation of epidermal growth factor-activated receptor activity Notch signaling pathway Notch receptor processing memory post-embryonic development protein transport L-glutamate transport choline transport synaptic vesicle targeting single organismal cell-cell adhesion protein processing cerebral cortex cell migration Cajal-Retzius cell differentiation dorsal/ventral neural tube patterning embryonic limb morphogenesis positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis beta-amyloid formation intracellular signal transduction regulation of phosphorylation amyloid precursor protein catabolic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of catalytic activity regulation of protein binding positive regulation of MAP kinase activity negative regulation of neuron apoptotic process skin morphogenesis positive regulation of transcription, DNA-templated regulation of synaptic plasticity thymus development neuron development skeletal system morphogenesis brain morphogenesis epithelial cell proliferation negative regulation of axonogenesis positive regulation of coagulation T cell receptor signaling pathway neuron apoptotic process negative regulation of ubiquitin-protein transferase activity smooth endoplasmic reticulum calcium ion homeostasis regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway regulation of resting membrane potential positive regulation of dendritic spine development calcium ion transmembrane transport negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway negative regulation of protein phosphorylation positive regulation of protein phosphorylation myeloid leukocyte differentiation negative regulation of protein kinase activity proteolysis calcium ion transport cellular calcium ion homeostasis autophagy apoptotic process cell adhesion regulation of epidermal growth factor-activated receptor activity brain development heart development learning or memory embryo development cerebral cortex development neurogenesis neuron differentiation forebrain development segmentation locomotion anagen cellular protein metabolic process positive regulation of protein kinase activity beta-amyloid metabolic process protein maturation
It has to be done as per old AB suggested Products section.
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