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Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (3L18)

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Datasheet
Protocols
Questions & Answers

Cite Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (3L18)

  • Antibody Testing Data (3)
Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
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Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
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Phospho-mTOR (Ser2448) Antibody (80596-1-RR100UL) in WB

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with (Product # 80596-1-RR100UL) (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (3L18)

Product Details

80596-1-RR100UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:50,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

3L18

Conjugate

Unconjugated Unconjugated Unconjugated
  • CoraLite 555
  • CoraLite 594
  • CoraLite Plus 488
  • CoraLite Plus 647
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; Tyrosine-protein kinase mTOR

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Gene Aliases: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

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UniProt ID: (Human) P42345, (Rat) P42346

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Entrez Gene ID: (Human) 2475, (Rat) 56718

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Function(s)
RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity protein kinase binding protein domain specific binding ribosome binding phosphoprotein binding drug binding protein dimerization activity
Process(es)
negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart morphogenesis heart valve morphogenesis regulation of glycogen biosynthetic process energy reserve metabolic process 'de novo' pyrimidine nucleobase biosynthetic process DNA repair protein phosphorylation response to stress cell cycle arrest signal transduction germ cell development brain development cell aging response to nutrient long-term memory visual learning post-embryonic development negative regulation of autophagy positive regulation of lamellipodium assembly positive regulation of gene expression positive regulation of myotube differentiation positive regulation of neuron maturation negative regulation of muscle atrophy cell growth macroautophagy negative regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development protein catabolic process positive regulation of actin filament polymerization T cell costimulation negative regulation of protein ubiquitination ruffle organization regulation of myelination cellular response to nutrient levels TOR signaling regulation of fatty acid beta-oxidation regulation of response to food response to insulin regulation of actin cytoskeleton organization social behavior multicellular organism growth growth wound healing response to cocaine regulation of GTPase activity response to amino acid response to morphine regulation of carbohydrate utilization positive regulation of nitric oxide biosynthetic process regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter protein autophosphorylation positive regulation of lipid biosynthetic process phosphatidylinositol-mediated signaling mRNA stabilization positive regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation positive regulation of peptidyl-tyrosine phosphorylation voluntary musculoskeletal movement positive regulation of stress fiber assembly negative regulation of NFAT protein import into nucleus positive regulation of protein kinase B signaling cardiac muscle cell development cardiac muscle contraction maternal process involved in female pregnancy positive regulation of glial cell proliferation positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to hypoxia regulation of brown fat cell differentiation regulation of membrane permeability regulation of cellular response to heat positive regulation of neuron death positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of eating behavior positive regulation of cholangiocyte proliferation positive regulation of sensory perception of pain negative regulation of cholangiocyte apoptotic process positive regulation of granulosa cell proliferation positive regulation of skeletal muscle hypertrophy negative regulation of iodide transmembrane transport double-strand break repair via homologous recombination regulation of carbohydrate metabolic process cell projection organization
It has to be done as per old AB suggested Products section.
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