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Invitrogen

Phospho-eIF4B (Ser422) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite Phospho-eIF4B (Ser422) Polyclonal Antibody

  • Antibody Testing Data (1)
Phospho-eIF4B (Ser422) Antibody in Western Blot (WB)
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Phospho-eIF4B (Ser422) Antibody in Western Blot (WB)
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Phospho-eIF4B (Ser422) Antibody (PA5-97393) in WB

Western blot analysis of Phospho-eIF4B (Ser422) in extracts of HeLa cells. Samples were incubated with Phospho-eIF4B (Ser422) Polyclonal antibody (Product # PA5-97393) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by EGF (100 ng/mL) for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-eIF4B (Ser422) Antibody in Western Blot (WB)
Phospho-eIF4B (Ser422) Polyclonal Antibody

Product Details

PA5-97393

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around S422 of human EIF4B (NP_0014082)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2809195

Product Specific Information

Immunogen sequence: TGSES; Positive Samples: HeLa

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609

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Gene Aliases: EIF-4B; EIF4B; PRO1843

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UniProt ID: (Human) P23588

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Entrez Gene ID: (Human) 1975

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Function(s)
nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding
Process(es)
nuclear-transcribed mRNA poly(A) tail shortening formation of translation preinitiation complex cytoplasmic translation translational initiation regulation of translational initiation eukaryotic translation initiation factor 4F complex assembly
It has to be done as per old AB suggested Products section.
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