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  • eIF4B Antibodies

Invitrogen

Phospho-eIF4B (Ser406) Recombinant Rabbit Monoclonal Antibody (23GB3905)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-eIF4B (Ser406) Recombinant Rabbit Monoclonal Antibody (23GB3905)

  • Antibody Testing Data (2)
Phospho-eIF4B (Ser406) Antibody in Western Blot (WB)
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Phospho-eIF4B (Ser406) Antibody in Western Blot (WB)
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Phospho-eIF4B (Ser406) Antibody (MA5-53662) in WB

Western Blot analysis of eIF4B. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with eIF4B recombinant monoclonal antibody (Product # MA5-53662) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-eIF4B (Ser406) Antibody in Western Blot (WB)
Phospho-eIF4B (Ser406) Antibody in Flow Cytometry (Flow)
Phospho-eIF4B (Ser406) Recombinant Rabbit Monoclonal Antibody (23GB3905)

Product Details

MA5-53662

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Flow Cytometry (Flow)

1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

23GB3905

Immunogen

A synthetic peptide of human Phospho-eIF4B (Ser406) (399-413AA).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3248133

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609

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Gene Aliases: 2310046H11Rik; AL024095; C85189; EIF-4B; Eif4a2; EIF4B; PRO1843

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UniProt ID: (Human) P23588, (Mouse) Q8BGD9

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Entrez Gene ID: (Human) 1975, (Rat) 300253, (Mouse) 75705

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Function(s)
nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding nucleic acid binding
Process(es)
translational initiation nuclear-transcribed mRNA poly(A) tail shortening formation of translation preinitiation complex cytoplasmic translation regulation of translational initiation eukaryotic translation initiation factor 4F complex assembly translation
It has to be done as per old AB suggested Products section.
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