Invitrogen

Phospho-c-Raf (Ser621) Recombinant Rabbit Monoclonal Antibody (3K8H3)

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Datasheet
Protocols
Questions & Answers
Phospho-c-Raf (Ser621) Recombinant Rabbit Monoclonal Antibody (3K8H3)

Product Details

MA5-35861

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3K8H3

Immunogen

A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (P04049)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849759

Product Specific Information

Immunogen sequence: SASEP

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-RAF; C-Raf proto-oncogene, serine/threonine kinase; cRaf; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le

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Gene Aliases: c-Raf; CMD1NN; CRAF; NS5; RAF; Raf-1; RAF1

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UniProt ID: (Human) P04049, (Rat) P11345

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Entrez Gene ID: (Human) 5894, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding metal ion binding protein heterodimerization activity receptor signaling protein activity Ras GTPase binding
Process(es)
MAPK cascade activation of MAPKK activity response to hypoxia stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process signal transduction activation of adenylate cyclase activity heart development cell proliferation negative regulation of cell proliferation cell differentiation platelet activation thyroid gland development negative regulation of protein complex assembly positive regulation of peptidyl-serine phosphorylation ion transmembrane transport somatic stem cell population maintenance regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch wound healing regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway thymus development face development death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell motility cellular glucose homeostasis positive regulation of protein phosphorylation intracellular signal transduction
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