Invitrogen

Phospho-TRKB (Tyr516) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Phospho-TRKB (Tyr516) Polyclonal Antibody

Product Details

PA5-105013

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NTRK2(Accession Q16620), corresponding to amino acid residues around phosphorylated Tyr516.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816486

Product Specific Information

Antibody detects endogenous levels of Trk B only when phosphorylated at Tyrosine 516.

Target Information

NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system. NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses. TrkB is involved in the development and maintenance of the nervous system. Moreover, Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. TrkB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for the TrkB gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AI848316; BDNF-tropomyosine receptor kinase B; BDNF/NT-3 growth factors receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP145TrkB; GP95 TrkB; GP95-TrkB; hyperphagia; kinase TrkB; neural receptor protein-tyrosine kinase (trkB); Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; neurotrophic tyrosine receptor kinase type 2; NTRK 2; OTTHUMP00000021573; OTTHUMP00000021574; OTTHUMP00000021576; OTTHUMP00000021577; Trk B; Trk-B; TrkB tyrosine kinase; Tropomyosin-related kinase B; tyrosine kinase receptor B

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Gene Aliases: GP145-TrkB; GP145-TrkB/GP95-TrkB; NTRK2; RATTRKB1; Tkrb; trk-B; TRKB; TRKB1

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UniProt ID: (Human) Q16620, (Mouse) P15209, (Rat) Q63604

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Entrez Gene ID: (Human) 4915, (Mouse) 18212, (Rat) 25054

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Function(s)
ATP binding receptor tyrosine kinase binding protein homodimerization activity neurotrophin binding brain-derived neurotrophic factor binding brain-derived neurotrophic factor-activated receptor activity nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity neurotrophin receptor activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ephrin receptor binding
Process(es)
vasculogenesis neuron migration protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development nervous system development neuromuscular junction development learning long-term memory feeding behavior positive regulation of cell proliferation positive regulation of gene expression positive regulation of neuron projection development glutamate secretion positive regulation of phosphatidylinositol 3-kinase signaling phosphorylation peptidyl-tyrosine phosphorylation regulation of metabolic process central nervous system neuron development cerebral cortex development cell differentiation neuron differentiation brain-derived neurotrophic factor receptor signaling pathway positive regulation of peptidyl-serine phosphorylation calcium-mediated signaling using intracellular calcium source neurotrophin signaling pathway mechanoreceptor differentiation regulation of GTPase activity regulation of MAPK cascade positive regulation of MAPK cascade negative regulation of neuron apoptotic process retinal rod cell development protein autophosphorylation regulation of neurotransmitter secretion oligodendrocyte differentiation peripheral nervous system neuron development positive regulation of axonogenesis regulation of dendrite development regulation of protein kinase B signaling positive regulation of synapse assembly positive regulation of synaptic transmission, glutamatergic retina development in camera-type eye long-term synaptic potentiation cellular response to amino acid stimulus positive regulation of glucocorticoid receptor signaling pathway negative regulation of anoikis positive regulation of protein phosphorylation inflammatory response aging circadian rhythm response to light stimulus response to auditory stimulus cellular response to tumor necrosis factor cellular response to brain-derived neurotrophic factor stimulus cellular response to nerve growth factor stimulus
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