Invitrogen

Phospho-TERT (Tyr707) Polyclonal Antibody

Name: Phospho-TERT (Tyr707) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-13048

1 Reference

View all (37) TERT antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Phospho-TERT (Tyr707) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

Phospho-TERT (Tyr707) Antibody (PA5-13048) in ICC/IF

Immunocytochemistry analysis of Phospho-TERT (Tyr707) in HeLa cells. Samples were incubated in Phospho-TERT (Tyr707) polyclonal antibody (Product # PA5-13048) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Phospho-TERT (Tyr707) Antibody in Dot Blot (DB)

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Immunogen image

Immunogen Image Phospho-TERT (Tyr707) Polyclonal Antibody

Product Details

PA5-13048

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:50

Flow Cytometry (Flow)

View 1 publication 1 publication

Dot blot (DB)

1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y707 of human TERT

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2201586

Target Information

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TERT inhibition leads to reduction of IL-6 expression induced by palmitic acid and interferes with the protective effects of tibolone in an astrocytic cell model.

Journal of neuroendocrinology

González-Giraldo Y,Garzón-Benitez AV,Forero DA,Barreto GE

PA5-13048 was used in Flow cytometry/Cell sorting to conclude that telomerase could play a dual role in these cells.

Thu Aug 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: HEST2; hTERT; Telomerase catalytic subunit; Telomerase reverse transcriptase; Telomerase-associated protein 2; TP2

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Gene Aliases: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TRT

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UniProt ID: (Human) O14746

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Entrez Gene ID: (Human) 7015

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Function(s)

tRNA binding transcription coactivator binding DNA binding telomerase activity telomerase RNA reverse transcriptase activity RNA binding RNA-directed DNA polymerase activity RNA-directed RNA polymerase activity protein binding nucleotidyltransferase activity telomeric DNA binding protein homodimerization activity metal ion binding telomerase RNA binding

Process(es)

telomere maintenance transcription, RNA-templated RNA-dependent DNA biosynthetic process telomere maintenance via telomerase mitochondrion organization negative regulation of gene expression DNA strand elongation positive regulation of Wnt signaling pathway production of siRNA involved in RNA interference regulation of protein stability positive regulation of protein binding RNA biosynthetic process positive regulation of hair cycle positive regulation of nitric-oxide synthase activity establishment of protein localization to telomere cellular response to hypoxia DNA biosynthetic process replicative senescence positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of production of siRNA involved in RNA interference positive regulation of protein localization to nucleolus beta-catenin-TCF complex assembly positive regulation of stem cell proliferation negative regulation of cellular senescence negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-TERT (Tyr707) Polyclonal Antibody

Phospho-TERT (Tyr707) Antibody (PA5-13048) in ICC/IF

Product Details

PA5-13048

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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