NeoBiotechnologies

Phospho-RNA Polymerase II CTD Repeat YSPTSPS (Ser5) Monoclonal Antibody (CTD 8A7)

View all (46) RNA pol II CTD antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

5430-MSM2-P1BX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Yeast

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

CTD 8A7

Immunogen

Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3'-5' exodeoxyribonuclease; 3'-5' exoribonuclease; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; Pol2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8

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Gene Aliases: DUN2; hRPB220; hsRPB1; N0825; POL2; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2; YNL262W

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UniProt ID: (Baker's yeast) P21951, (Human) P24928

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Entrez Gene ID: (Baker's yeast) 855459, (Human) 5430

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Function(s)
nucleotide binding nucleic acid binding DNA binding double-stranded DNA binding single-stranded DNA binding DNA-directed DNA polymerase activity zinc ion binding single-stranded DNA 3'-5' exodeoxyribonuclease activity transferase activity nucleotidyltransferase activity metal ion binding iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding RNA polymerase II activity DNA-directed RNA polymerase activity RNA-directed RNA polymerase activity protein binding ubiquitin protein ligase binding poly(A) RNA binding
Process(es)
DNA synthesis involved in DNA repair nucleobase-containing compound metabolic process DNA replication DNA-dependent DNA replication leading strand elongation base-excision repair nucleotide-excision repair, DNA gap filling double-strand break repair via nonhomologous end joining mitotic sister chromatid cohesion intra-S DNA damage checkpoint mitotic DNA replication checkpoint gene conversion error-prone translesion synthesis DNA replication proofreading heterochromatin organization involved in chromatin silencing mRNA splicing, via spliceosome transcription, RNA-templated transcription-coupled nucleotide-excision repair DNA-templated transcription, termination regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA positive regulation of RNA splicing somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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