Invitrogen

Phospho-NPM1 (Ser4) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Phospho-NPM1 (Ser4) Polyclonal Antibody

Product Details

PA5-106194

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NPM1(Accession P06748), corresponding to amino acid residues around phosphorylated Ser4.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817592

Product Specific Information

Antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Ser4.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; nucleolar protein B23.1; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128

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Gene Aliases: B23; B23NP; NO38; NPM; NPM1

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UniProt ID: (Human) P06748, (Rat) P13084, (Mouse) Q61937

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Entrez Gene ID: (Human) 4869, (Rat) 25498, (Mouse) 18148

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Function(s)
transcription coactivator activity RNA binding protein kinase inhibitor activity protein binding protein kinase binding Tat protein binding histone binding protein homodimerization activity ribosomal large subunit binding ribosomal small subunit binding poly(A) RNA binding protein heterodimerization activity NF-kappaB binding unfolded protein binding DNA binding phosphatidylinositol-3,4,5-trisphosphate binding rRNA binding enzyme binding nucleic acid binding protein N-terminus binding
Process(es)
DNA repair nucleosome assembly intracellular protein transport nucleocytoplasmic transport response to stress DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest centrosome cycle signal transduction cell aging protein localization positive regulation of cell proliferation negative regulation of cell proliferation negative regulation of centrosome duplication viral process regulation of endodeoxyribonuclease activity CENP-A containing nucleosome assembly ribosome assembly negative regulation of apoptotic process negative regulation of protein kinase activity by regulation of protein phosphorylation positive regulation of translation positive regulation of transcription, DNA-templated regulation of centriole replication positive regulation of NF-kappaB transcription factor activity protein oligomerization regulation of endoribonuclease activity regulation of eIF2 alpha phosphorylation by dsRNA positive regulation of cell cycle G2/M phase transition ribosomal large subunit export from nucleus ribosomal small subunit export from nucleus rRNA export from nucleus cell volume homeostasis regulation of centrosome duplication positive regulation of centrosome duplication cell growth positive regulation of cellular biosynthetic process protein destabilization ribosomal large subunit biogenesis ribosomal small subunit biogenesis positive regulation of catalytic activity regulation of DNA damage response, signal transduction by p53 class mediator regulation of neuron apoptotic process positive regulation of DNA replication positive regulation of protein kinase activity negative regulation of mRNA splicing, via spliceosome positive regulation of DNA metabolic process protein homooligomerization regulation of cell cycle
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