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  • LIMK1 Antibodies

Invitrogen

Phospho-LIMK1 (Tyr507, Thr508) Polyclonal Antibody

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View all (58) LIMK1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Phospho-LIMK1 (Tyr507, Thr508) Polyclonal Antibody

  • Antibody Testing Data (1)
Phospho-LIMK1 (Tyr507, Thr508) Antibody in Western Blot (WB)
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Phospho-LIMK1 (Tyr507, Thr508) Antibody in Western Blot (WB)
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Phospho-LIMK1 (Tyr507, Thr508) Antibody (44-1076G) in WB

Antibody-Peptide Competition. Extracts of Jurkat cells treated with 5 nM SDF-1alpha for 20 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the LIMK1/2 (pYpT507/508) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-LIMK1 (Tyr507, Thr508) Antibody in Western Blot (WB)
Phospho-LIMK1 (Tyr507, Thr508) Polyclonal Antibody

Product Details

44-1076G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human LIMK1 that contains tyrosine 507 and threonine 508 (tyrosine 504 and threonine 505 in LIMK2). The LIMK1 sequence is conserved in mouse and rat, and the LIMK2 sequence is conserved in mouse, rat, and chicken.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533567

Target Information

LIMK1 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK1 has also been implicated in brain development. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.11.1; kinase LIMK1; LIM domain kinase 1; lim kinase 1; LIM motif-containing protein kinase; LIMK-1

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Gene Aliases: LIMK; LIMK-1; LIMK1

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UniProt ID: (Human) P53667

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Entrez Gene ID: (Human) 3984

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding zinc ion binding heat shock protein binding protein heterodimerization activity
Process(es)
protein phosphorylation signal transduction Rho protein signal transduction nervous system development actin cytoskeleton organization positive regulation of actin filament bundle assembly Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of axon extension negative regulation of ubiquitin-protein transferase activity positive regulation of stress fiber assembly
It has to be done as per old AB suggested Products section.
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