Invitrogen

Phospho-IKK beta (Tyr188) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Phospho-IKK beta (Tyr188) Polyclonal Antibody

Product Details

PA5-104693

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IKBKB(Accession O14920), corresponding to amino acid residues around phosphorylated Tyr188.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816166

Product Specific Information

Antibody detects endogenous levels of IKK beta only when phosphorylated at Tyrosine 188.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-2; IKK-B; IKK-beta; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; Serine/threonine protein kinase IKBKB

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Gene Aliases: AI132552; AIM-1; IKBKB; IKK-2; IKK-beta; IKK2; IKK[b]; IKKB; IKKbeta; IMD15; NFKBIKB

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UniProt ID: (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

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Entrez Gene ID: (Human) 3551, (Mouse) 16150, (Rat) 84351

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding IkappaB kinase activity protein kinase binding protein homodimerization activity protein heterodimerization activity scaffold protein binding nucleotide binding receptor signaling protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
B cell homeostasis protein phosphorylation I-kappaB phosphorylation positive regulation of cell proliferation positive regulation of sodium ion transport phosphorylation signal transduction by protein phosphorylation neuron projection development serine phosphorylation of STAT protein positive regulation of transcription from RNA polymerase II promoter positive regulation of NF-kappaB transcription factor activity cellular response to tumor necrosis factor positive regulation of neuron death positive regulation of cation channel activity stimulatory C-type lectin receptor signaling pathway skeletal muscle contraction inflammatory response I-kappaB kinase/NF-kappaB signaling response to virus response to toxic substance regulation of tumor necrosis factor-mediated signaling pathway positive regulation of neuron projection development cortical actin cytoskeleton organization response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway negative regulation of myosin-light-chain-phosphatase activity TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway regulation of phosphorylation response to drug negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of transcription, DNA-templated T cell receptor signaling pathway stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway interleukin-1-mediated signaling pathway response to fatty acid establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly
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